Application

Research CategoryStem Cell Research

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Product Source: E. coli

Epidermal growth factor (EGF) is the founding member of the EGF family that also includes TGFα, amphiregulin (AR), betacellulin (BTC), epiregulin (EPR), heparinbinding EGF like growth factor (HB-EGF), epigen, and the neuregulins (NRG)1-6. EGF is a potent growth factor that stimulates the proliferation of various epidermal and epithelial cells. Additionally, EGF has been shown to inhibit gastric secretion, and to be involved in wound healing. EGF signals through a receptor known as c-erbB, which is a class I tyrosine kinase receptor. This receptor also binds with TGF-α and VGF (vaccinia virus growth factor). Recombinant human EGF is a 6.2 kDa globular protein containing 53 amino acid residues including 3 intramolecular disulfide-bonds.

Physical form

Product is filtered through a 0.2 micron filter before lyophilization.

Recombinant animal free human EGF is manufactured using all non-animal reagents

Quality

Bioactivity assay: The ED50 was determined by a cell proliferation assay using balb/c 3T3 cells is ≤0.1 ng/mL, corresponding to a specific activity of ≥1 x 107 units/mg.

Specificity

Cross ReactivtyCow, Hamster, Monkey, Mouse, Pig, Rabbit, Rat

Storage and Stability

Store at -20°C for up to 6 months from date of receipt.